Expression of baculovirus recombinant HPV16L1 and L2 capsid proteins in insect cells

魏兰兰; 韩立群; 任皎; 田厚文; 骆卫锋; 陆 振华; 谷鸿喜; 阮力

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Expression of baculovirus recombinant HPV16L1 and L2 capsid proteins in insect cells

魏兰兰韩立群任皎田厚文骆卫锋陆振华谷鸿喜阮力

Year: 2001 Journal: 中华实验和临床病毒学杂志 Vol: 15 (4)Pages: 309-313

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Abstract

目的在昆虫细胞中表达人乳头瘤病毒16型(HPV16)结构蛋白,为HPV16型预防性基因工程亚单位疫苗的研究以及诊断试剂的研制奠定基础.方法将目的基因克隆至杆状病毒转移载体,该重组质粒DNA与线性化的杆状病毒DNA共转染昆虫细胞Sf 9,通过同源重组获得重组杆状病毒,用SDS-PAGE凝胶电泳和Western blot法检测重组子在昆虫细胞中表达的目的蛋白.结果获得了稳定高效表达HPV16结构蛋白的重组杆状病毒,L1和L2蛋白的相对分子质量分别为57 000和97 000,L1蛋白的表达产量约占昆虫细胞总体蛋白的25%～30%.结论人乳头瘤病毒16型结构蛋白可在昆虫细胞内高效表达.

Keywords:

Capsid Recombinant DNA Baculoviridae Cell biology Insect Biology Virus-like particle Recombinant virus Spodoptera Virology Physics Virus Genetics Gene Ecology

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Viral Infectious Diseases and Gene Expression in Insects

Life Sciences → Biochemistry, Genetics and Molecular Biology → Molecular Biology

Expression of baculovirus recombinant HPV16L1 and L2 capsid proteins in insect cells

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