CVD法による窒化ニオブ薄膜の合成(2. 気相反応法)( 新技術によるセラミックスの合成と評価(I))

Abstract

Graphene oxide (GO) and silver-graphene oxide (Ag-GO) are used in various fields, such as biotechnology and environmental engineering, due to their unique material properties, including hydrophilicity, high surface area, mechanical strength, and antibacterial activity. With the increase in the usage of such nanomaterials, they are likely to enter the aquatic environment during the manufacturing process, product use, and disposal. However, the effects of GO and Ag-GO on aquatic microbial activities are not well understood. In this study, we aimed to determine the effects of GO and Ag-GO on the aquatic microbial communities inhabiting a river and a lake located in Seoul, South Korea. Unfiltered natural surface water samples were exposed to GO and Ag-GO at a final concentration of 10 to 100 mg L^-1 for 48 h. The activity of leucine aminopeptidase was significantly lowered within 1 h of GO and Ag-GO treatments and nitrification rate was significantly lowered. An increase in intracellular lactate dehydrogenase levels of up to 5% was observed in natural waters under GO and Ag-GO treatments compared to the control (0%), indicating cell membrane damage. In addition, generation of intracellular reactive oxygen species increased up to 184% under 100 mg GO L^-1 and 102% under 100 mg Ag-GO L^-1 treatment compared to the control (0%). Our results indicate that the activities of microorganisms inhabiting natural surface waters may have been inhibited by oxidative stress and cell membrane damage induced by GO and Ag-GO. We believe that our results may contribute to the development of regulatory guidelines on the release of emerging engineered nanomaterials to the environment.