Facile Preparation of Hemin-functionalized Electrochemically Reduced Graphene Oxide Nanocomposite for H2O2 Biosensing

Abstract

A Hemin-f unctionalized electrochemically reduced graphene oxide (H-ERGO) nanocomposite was prepared for the construction of a nonenzymatic H 2 O 2 biosensor.H-ERGO was directly modified on the electrode surface without using a binder by electrochemical reduction.Detailed physical and electrical characterizations of H-ERGO were conducted by scanning electron microscopy, UV-vis absorption spectroscopy, and electrochemical impedance spectroscopy (EIS).Taking advantage of the superior catalytic properties of H-ERGO, we applied the proposed biosensor to H 2 O 2 analysis.Under optimized conditions, the H-ERGObased biosensor exhibited a wide linear range from 25 to 8850 and 8850 to 28850 μM, with a detection limit (S/N = 3) of 8.33 μM for H 2 O 2 determination.Furthermore, it was also successfully applied to detect H 2 O 2 in milk samples with recoveries ranging from 94.6 to 98.1%.