The first complete plastome sequence from the family Cardiopteridaceae, Gonocaryum lobbianum (Miers) Kurz

Abstract

In this study, we determined the complete plastome sequence of Gonocaryum lobbianum (Miers) Kurz (Cardiopteridaceae) (NCBI acc. no. MK390345). This is the first reported complete plastome sequence from the family Cardiopteridaceae of the order Aquifoliales. The complete plastome of G. lobbianum is 158,905 bp in length and consists of a large single copy of 87,705 bp and a small single copy of 19,346 bp, separated by two inverted repeats of 25,927 bp. The plastome contains 112 genes, of which 78 are protein-coding genes, 30 are tRNA genes, and four are rRNA genes. The rpoA gene was pseudogenized because of deletion and following frame-shift mutations. We also confirmed the pseudonization of rpoA gene using PCR and conventional Sanger sequencing methods. Sixteen genes contain one intron and two genes have two introns. The average A-T content of the plastome is 62.7%. A total of 73 simple sequence repeat loci were identified within the genome. Phylogenetic analysis revealed that G. lobbianum is a sister group of Aquifoliaceae-Helwingiaceae with 100% bootstrap value. The Aquifoliales clade is a sister group to the Apiales-Asterales-Dipsacales clade with 100% bootstrap support.