JOURNAL ARTICLE

Real-Time Confocal Fluorescence Lifetime Imaging Microscopy (FLIM) for Spectroscopic Sensing of Cancer Tissues

Abstract

We demonstrated GPU accelerated real-time confocal fluorescence lifetime imaging microscopy (FLIM) based on the analog mean-delay (AMD) method. The frame rate of our system was demonstrated to be 13 fps for a 200 × 200 pixel image when observing maize vascular tissue. This system can be utilized for studying spectroscopic characteristics of cancer tissues.

Keywords:
Confocal Fluorescence-lifetime imaging microscopy Confocal microscopy Fluorescence Microscopy Materials science Fluorescence microscope Frame rate Microscope Pixel Optics Biomedical engineering Physics Medicine

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Citation History

Topics

Advanced Fluorescence Microscopy Techniques
Life Sciences →  Biochemistry, Genetics and Molecular Biology →  Biophysics
Optical Imaging and Spectroscopy Techniques
Health Sciences →  Medicine →  Radiology, Nuclear Medicine and Imaging
Photoacoustic and Ultrasonic Imaging
Physical Sciences →  Engineering →  Biomedical Engineering
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