Development of Titania-Integrated Silica Cell Walls of the Titanium-Resistant Diatom, Fistulifera solaris

Abstract

We report the biological synthesis of titania that is integrated into the silica-based cell walls of a titanium-resistant diatom, Fistulifera solaris. Titania is deposited across the diatom cell walls by simply incubating F. solaris in a culture medium containing a high concentration (2 mM) of a water-soluble organo-titanium compound, titanium(IV) bis(ammonium lactato) dihydroxide (TiBALDH) that would otherwise inhibit the growth of other diatom species. Furthermore, we genetically engineered the interfaces of the diatom cell walls with a titanium-associated peptide, which subsequently increased the Ti/Si atomic ratio by more than 50% (i.e., from 6.2 ± 0.2% to 9.7 ± 0.5%, as identified by inductively coupled plasma-atomic emission spectrometry). The titanium content on the F. solaris silica cell walls is one of the highest reported to date, and comparable to that of chemically synthesized TiO₂-silica composites. Subsequent thermal annealing at 500 °C in air converted the cell wall-bound titania to nanocrystalline anatase TiO₂, a highly photocatalytically active phase. We propose that incubation of the titanium-resistant F. solaris with TiBALDH as demonstrated in this study could be a promising bioprocess toward the scalable synthesis of TiO₂. In addition, the genetic engineering we used to modulate the surface properties of diatom silica cell walls could be extended to synthesize controlled nanomaterials for multiple applications including bioremediation, water purification, and energy conversion/storage.