Chromolaena Odorata Leaf Extract Hand Sanitizer

Abstract

Hygiene is imperative to maintaining health and preventing disease. Hand washing with soap and water helps in the prevention of transmission of diseases, a measure to prevent nosocomial infection in the healthcare setting where opportunistic bacterial like Staphylococcus aureus and Escherichia coli are acquired. This study aimed to formulate liquid solution containing the leaf extract obtained from Chromolaena odorata (L.) King & Robinson family Asteraceae, that has potential antibacterial property. The antibacterial property of the formulation was tested employing the disc diffusion method against Staphylococcus aureus (gram positive) and Escherichia coli (gram negative). The diameters of the zones of inhibition were measured. The tests of the different concentrations of the formulated C. odorata hand sanitizer solution were carried in one run. The three different concentrations were 1.0 gram, 0.75g, and 0.5g of ethanolic extracts per 100-milliliter preparation. The positive control used was ampicillin, and the negative control was isopropyl alcohol. The tests revealed that there were no zones of inhibition observed against the test organisms on the three different concentrations. The formulated Chromolaena odorata leaf extract hand sanitizer liquid solution containing 1.0 g, 0.75g, and 0.5g of ethanolic extracts has no antibacterial property against Staphylococcus aureus and Escherichia Coli. Keywords : hand sanitizer, Chromolaena odorata, antibacterial property