Muhammad Faiz RamliAri LegowoSyariful Syafiq Shamsudin
Local natural product extracts has been gaining great focus from research and \ntherapeutic aspect. Similarly, the outcome of studies on one of nation’s renowned \nhoney Tualang (TH) (Koompassia excelsa) has profoundly anchored its board \naptitude in anti-inflammatory, anti-oxidant and anti-microbial properties. This \nresearch focuses on the study of the immunomodulatory properties of TH in \nBALB/c. TH was orally administered daily for 14 days to BALB/c male mice \n(5/group) in dose ranging from 0.5 g/kg, 1.5 g/kg and 3.0 g/kg namely Low group, \nMedium group and High group. Pre-treatment and post-treatment body weights were \nmeasured. Upon sacrificing, the spleen was weighed and then homogenized. The \nsplenocytes were stained with various surface markers antibodies namely \nCD3+/CD4+(T helper), CD3+/CD8+(T cytotoxic), CD14+ (macrophage) and CD19+ \n(B lymphocyte) and immune cell populations were obtained by using flow \ncytometer. On the other hand, proliferation assay of splenocytes were done by using \nCellTiter 96® AQueous One Solution Cell Proliferation Assay (MTS). Body weight \nin Control, Medium and High groups showed increment after the treatment but body \nweight of mice in Low group decreased upon completion of TH treatment. BALB/c \nmice treated with TH showed increased populations of CD3+/CD4+, CD3+/CD8+, \nCD14+ and CD19+ compared to control group. Proliferation analysis of splenocytes \nas well as spleen weight obtained from TH-treated mice also showed an increment. \nThe results revealed the immunostimulant potential of TH in mice by enhancing \nlymphocyte populations especially T helper (CD3+/CD4+), cytotoxic (CD3+/CD8+) \nand B (CD19+) cells.
Mingjie LiuXianhao WangAnjian ZhouXiuyuan FuYiwei MaChanghao Piao
C. Y. WangYuh-Lin ChangShan Juan XieXiaobao YangYadong TianWei SunJunyan Hu
Tingting GuoQingzeng SongYongjiang XueFei Qiao
Jie LiuJinzong CuiMao YeXiatian ZhuSong Tang