Enzyme-catalysed Biodiesel Production from Edible and Waste Cooking Oils

Abstract

Biodiesel synthesis was performed as transesterification of edible and waste cooking sunflower oil catalysed by free lipase from Thermomyces lanuginosus (Lipolase 100L). Experiments were performed at three different temperatures (T = 40, 50 and 60 °C) as one-step and four-step reactions with methanol. The highest fatty acids methyl esters (FAME) content (C = 95 %) was achieved in the one-step transesterification reaction of edible sunflower oil performed at 40 °C.