Rapid detection of tenuazonic acid in cereal and fruit juice using a lateral-flow immunochromatographic assay strip

Abstract

A lateral-flow immunochromatographic assay (ICA) strip was developed for determination of tenuazonic acid (TeA) using both semi-quantitative and quantitative methods. The ICA strip was based on produced anti-TeA monoclonal antibody, and could be used for TeA analysis in cereal and fruit juice, with visual limits of detection of 1600 ng/g and 400 ng/mL, and cut-off values of 6400 ng/g and 3200 ng/mL, respectively. The calculated limits of detection values were 126.4 ng/g and 65.3 ng/mL for wheat and apple juice samples, respectively. The recovery rates ranged from 90% to 129%. In summary, the developed strip was effective for TeA detection and would be suitable for on-site detection and rapid screening of samples.