JOURNAL ARTICLE
Multiplexed fluorescence lifetime image with Fourier excitation-emission spectroscopy
Year: 2011 Journal: Optics in the Life Sciences Vol: 35 Pages: BWC3-BWC3
Abstract
We report a Fourier transform lifetime spectroscopy method simultaneously measures fluorescence lifetime and intensity on multiple excitation and emission channels in 46 microseconds. The technique will allow fast imaging study of multicolor Förster resonance energy transfer.
Keywords:
Fourier transform spectroscopy Excitation Fluorescence Spectroscopy Fourier transform Microsecond Fluorescence spectroscopy Förster resonance energy transfer Materials science Optics Fourier transform infrared spectroscopy Multiplexing Emission spectrum Imaging spectroscopy Energy transfer Nuclear magnetic resonance Atomic physics Physics Computer science Spectral line Telecommunications
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Topics
Advanced Fluorescence Microscopy Techniques
Life Sciences → Biochemistry, Genetics and Molecular Biology → Biophysics
Photoacoustic and Ultrasonic Imaging
Physical Sciences → Engineering → Biomedical Engineering
Photoreceptor and optogenetics research
Life Sciences → Neuroscience → Cellular and Molecular Neuroscience
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