Chemical composition and antibacterial activities of algerian Pinus halepensis Mill and Pinus maritima essential oils

Abstract

The chemical composition of the essential oils obtained from the needles of the two species Pinus maritima harvested from two areas, Hadada (site 1) and Tonga forests (site 2) in the National Park of El Kala, (North East of Algeria) and Pinus halepensis Mill. collected from Mellah Lake forest (site 3) and Zaarouria forest (site 4) (Souk Ahras, internal Algeria). The yield is respectively 0.27%, 0.18%, 0.81% and 0.3%. The essential oils investigated by gas chromatography coupled with the masse spectrometry type schimadzu equiped with FID, GC-MS analysis revealed the presence of Pinus maritima oils from (site 1). The major ones are Alpha-pinene (40.31%), Beta-caryophyllene (28.54%), and caryophyllene oxide (13.42%). In (site 2), the major compounds are Beta-cryophyllene (35.99%), Alpha-pinene (20.63%), Alpha-bulnesene (15.93%). The oil of Pinus halepensis Mill of (site 3) is composed mainly of Alpha-humulene (31.89%), Alpha-pinene (24.41%), Delta3-carene (19.38%), while that of (site 4) consists primarily of Aromadendrène (32.3%), Alpha-pinene (21.79%), Alpha-terpinolene (9.78%). The biological activity of essential oils (crude and diluted) tested against Escherichia coli; Staphylococcus aureus and Pseudomonas aeruginosa have showed antibacterial activities.