Comparative Evaluation of Various Methods for Detecting Antibody to Aujeszky's Disease Virus in Swine

Abstract

A total of 924 serum samples from swine in 1990 were examined by indirect immunoperoxidase plaque staining (IIPS) test, complement-dependent neutralization test (CNT), enzyme-linked immunosorbent assay (ELISA) and latex agglutination (LA) tests. Agreement among these methods for the detection of positive and negative sera was expressed as percentage of the total number of sera examined. Detection of the antibody by IIPS was compared with that by CNT, LA and ELISA. IIPS and CNT showed 99.6% agreement. IIPS and ELISA or LA indicated 93.7% and 93.3% agreement, respectively. Agreement of ELISA with the other methods ranged from 93.6% to 95.3%.Detection of the antibody by LA was compared with that of CNT, IIPS and ELISA. Agreement of LA with CNT, IIPS and ELISA was 93.1%, 93.3% and 95.3%, respectively.Definite results were obtained by CNT and IIPS, while the results for 12 and 35 of the 924 serum samples were ambiguous for ELISA and LA, respectively. Based on the present data, IIPS and CNT may be concluded to be the most reliable methods for conducting serodiagnosis of Aujeszky's disease in swine.