Preparation and hemocompatibility of electrospun O‐carboxymethyl chitosan/PVA nanofibers

Abstract

ABSTRACT Chitosan was deacetylated and carboxymethylated to prepare O‐carboxymethyl chitosan (CMC) for further electrospinning. CMC was characterized using FTIR, NMR, and chemical titration, indicating a degree of carboxymethylation of 51.4%. CMC was electrospun together with poly(vinyl alcohol) (PVA) to prepare membranes composed of nanofibers. The electrospinning conditions were optimized. The CMC/PVA membrane obtained at the conditions of 15.2 g/mL CMC 50 mL, 8 g/mL PVA 5 mL, 25 kV, and a distance of 23 cm, had nanofibers without beads, with diameters of 70–200 nm. The mats were crosslinked by glutaraldehyde before platelet adhesion measurement. The nanofibrous structure remained after crosslinking while the wettability decreased. CMC/PVA mats with higher CMC amount and fewer beads, had fewer adherent platelets and less platelets aggregation showing better hemocompatibility. © 2016 Wiley Periodicals, Inc. J. Appl. Polym. Sci. 2016 , 133 , 43565.