Multiplex PCR assay for simultaneous detection of six major bacterial pathogens of rice

Zhouqi Cui; Mohammad Reza Ojaghian; Tao Zheng; Kaleem U. Kakar; Jiamei Zeng; Wenjun Zhao; Y. P. Duan; C. M. Vera Cruz; Bin Li; Bo Zhu; G. L. Xie

doi:10.1111/jam.13094

JOURNAL ARTICLE

Multiplex PCR assay for simultaneous detection of six major bacterial pathogens of rice

Zhouqi Cui Mohammad Reza Ojaghian Tao Zheng Kaleem U. Kakar Jiamei Zeng Wenjun Zhao Y. P. Duan C. M. Vera Cruz Bin Li Bo Zhu G. L. Xie

Year: 2016 Journal: Journal of Applied Microbiology Vol: 120 (5)Pages: 1357-1367 Publisher: Oxford University Press

DOI: 10.1111/jam.13094

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Abstract

This study is the first report of a method allowing simultaneous detection of six major rice pathogens. The ability to use crude extracts from plants without bacterial isolation or DNA extraction enhances the value of this mPCR technology for rapid detection and aetiological/epidemiological studies.

Keywords:

Multiplex polymerase chain reaction Multiplex Biology Microbiology Polymerase chain reaction Bacteria Gene Genetics

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Plant Pathogenic Bacteria Studies

Life Sciences → Agricultural and Biological Sciences → Plant Science

Plant-Microbe Interactions and Immunity

Life Sciences → Agricultural and Biological Sciences → Plant Science

Genomics and Phylogenetic Studies

Life Sciences → Biochemistry, Genetics and Molecular Biology → Molecular Biology

Multiplex PCR assay for simultaneous detection of six major bacterial pathogens of rice

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Citation History

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