Immobilized Enzyme Gox with Ferrite CoFe₂O₄ and Hybrid CoFe₂O₄/APTS

Abstract

This work aims to evaluate the immobilization of glucose oxidase (GOx) with CoFe 2 O 4 nanoparticles and hybrids CoFe 2 O 4 /APTS. The nanoparticles were synthesized by combustion reaction and silanized using 3-aminopropyltrimethoxysilane (APTS). The samples were characterized by XRD, FTIR, magnetic measurements and immobilization of GOx. The results indicated single phase of spinel CoFe 2 O 4 and the addition of APTS did not alter the structure of the ferrite. The characteristic bands of spinel and characteristic bands were observed in the silane silanized sample proving the silanization of nanoparticles of CoFe 2 O 4 . The CoFe 2 O 4 nanoparticles and the hybrid had saturation magnetization of 58.0 and 53.0 emu/g and coercivity of 1.14 kOe and 1.15, indicating that the silanization does not interfere with the magnetism of the particles. The immobilized GOx was obtained with values of 0.047 and 0.050 mg/g efficiency of 84.86 and 80.30% for CoFe 2 O 4 /APTS and CoFe 2 O 4 .