GFP-Based Expression Screening of Membrane Proteins in Insect Cells Using the Baculovirus System

Nien‐Jen Hu; Heather Rada; Nahid Rahman; Joanne E. Nettleship; Louise E. Bird; So Iwata; David Drew; Alexander D. Cameron; Raymond J. Owens

doi:10.1007/978-1-4939-2230-7_11

JOURNAL ARTICLE

GFP-Based Expression Screening of Membrane Proteins in Insect Cells Using the Baculovirus System

Nien‐Jen Hu Heather Rada Nahid Rahman Joanne E. Nettleship Louise E. Bird So Iwata David Drew Alexander D. Cameron Raymond J. Owens

Year: 2014 Journal: Methods in molecular biology Vol: 1261 Pages: 197-209 Publisher: Springer Science+Business Media

DOI: 10.1007/978-1-4939-2230-7_11

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Keywords:

Green fluorescent protein Fusion protein Recombinant DNA Membrane protein Vesicle-associated membrane protein 8 Fluorescence Protein purification Protein expression Cell biology Membrane Biology Chemistry Biochemistry Molecular biology Gene

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Viral Infectious Diseases and Gene Expression in Insects

Life Sciences → Biochemistry, Genetics and Molecular Biology → Molecular Biology

Receptor Mechanisms and Signaling

Life Sciences → Biochemistry, Genetics and Molecular Biology → Molecular Biology

Neurobiology and Insect Physiology Research

Life Sciences → Neuroscience → Cellular and Molecular Neuroscience

GFP-Based Expression Screening of Membrane Proteins in Insect Cells Using the Baculovirus System

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