Pulsed Amperometric Detection of Carbohydrates in Fruit Juices Following High Performance Anion Exchange Chromatography

Abstract

Pulsed amperometric detection following liquid chromatography wasused for the determination of carbohydrates in fruit juices.Carbohydrates were separated isocratically using 100 mM NaOH on analkaline-tolerant anion-exchange column. Pulsed waveforms weregenerated using ChromGraph®software with the BAS DA-5 interfaceand LC-4C electrochemical detector. Juice samples were diluted,injected, and detected directly at a gold electrode following separation.Three sugars emerged as prominent and common to fruit juices bottledfrom concentrate: glucose, fructose, and sucrose. Detection of thesecompounds yielded detection limits of 0.7 ng (0.2 µM), 1.4 ng (0.4 µM),and 4.1 ng (0.6 µM) for glucose, fructose, and sucrose respectively. Thisapplications paper describes quantitation of these sugars in fruit juicesamples.