Polyaniline/Single‐Walled Carbon Nanotubes Composite Based Triglyceride Biosensor

Abstract

Abstract Nanocomposite film comprising of polyaniline (PANI) and single walled carbon nanotubes (SWCNT) has been fabricated onto indium‐tin‐oxide (ITO) coated glass plate using electrophoretic technique. Co‐immobilization of glycerol dehydrogenase (GDH) and lipase (LIP) has been done via N ‐ethyl‐ N′ ‐(3‐dimethylaminopropyl) carbodiimide and N ‐hydroxysuccinimide chemistry to explore its application for triglyceride (tributyrin) sensing. Response studies have been done using linear sweep voltammetry revealing that LIP‐GDH/PANI‐SWCNT‐TB/ITO bioelectrode can detect tributyrin in the range of 50 to 400 mg dL −1 with low Michaelis–Menten constant of 1.138 mM, improved response time of 12 s, high sensitivity as 4.28×10 −4 mA mg −1 dL and storage stability of about 13 weeks.