The promoter of Saccharomyces cerevisiae FBA1 gene contains a single positive upstream regulatory element

Abstract

The glycolytic enzyme fructose 1,6‐bisphosphate aldolase is encoded by the FBA1 gene of Saccharomyces cerevisiae . Transcription of aldolase gene is not regulated by glucose and high levels of expression have been observed also during growth on nonfermentable carbon source. A FBA1::lacZ gene fusion was constructed and a deletion analysis demonstrated the presence of a unique cis ‐acting positive upstream element (UAS) required for high levels of FBA1 expression. This element is located between positions −550 and −440 upstream of the aldolase open reading frame and it contains sequences known to constitute the binding sites for the multifunctional proteins RAP1 and ABF1 and two TTCC motifs.