Cell response to Electrospun PVA and PVA/Chitosan nanofibers

Abstract

PVA and chitosan have been used for many tissue engineering applications. However, most of the studies use only single polymer to fabricate nanofibers. Therefore, the purpose of this study is to fabricate PVA and PVA/Chitosan nanofiber scaffold for potential tissue engineering applications. The nanofrous scaffold was produced via electrospinning. NIH 3T3 fibroblast cells were seeded onto scaffolds for different time points. The attached fibroblast cells appeared round shape on the PVA nanofiber scaffold and spindle shape on the PVA/Chitosan nanofiber scaffold. The result shows that the fibroblast exhibit good cell viability and gene expression on the PVA/Chitosan nanofiber scaffold. Therefore, PVA/Chitosan nanofiber scaffold could be the better choice for further applications.