Method Development and Validation for Simultaneous Estimation of Atenolol and Nifedipine in Pharmaceutical Dosage Forms by RP-HPLC

Abstract

A simple precise and economical reverse phase high performance liquid chromatographic method has been developed and validated for the estimation of atenolol and nifedipine simultaneously in combined dosage form. The method was developed using agilent ODS C18 column with a mobile phase constituting of methanol:acetonitrile:phosphate buffer(60:20:20) final adjusted to pH 3.0 with o-phosporic acid at a flow rate of 1.0ml/min and detection was carried out at 235nm.. The selected chromatographic conditions were found to effectively separate atenolol (Rt: 2.80 min) and Nifedipine (Rt: 4.40 min) having a resolution of 12.307. The developed method was validated for linearity, accuracy, precision, LOD, LOQ, robustness and for system suitability parameters as per ICH guidelines. Linearity for atenolol and nifedipine were found in the range of 5-25ig/ml and 210ig/ml, respectively. The percentage recoveries for atenolol and nifedipine ranged from 99.38100.56% and 99.16-99.71%, respectively. The proposed method could be used for routine analysis of atenolol and nifedipine in their combined dosage forms.