Glucose oxidase electrodes based on microstructured polypyrrole films

Abstract

Abstract Highly sensitive glucose oxidase (GOD) electrodes were fabricated on the basis of microstructured polypyrrole (PPy) films. The microstructures of the PPy films had a morphology like cups and were arranged in a density of approximately 4000 units/cm 2 . GOD was immobilized in microstructured PPy films coated on a Pt or stainless steel (SS; AISI 321) substrate electrode. The GOD/PPy/Pt electrode showed a linear response to glucose concentrations in the range of 0–17 m M at a potential of 0.4 V (vs a saturated calomel electrode). Its sensitivity was measured to be approximately 660 nA/(m M cm 2 ) at 15°C, and the response time ( t 95% ) was approximately 20 s. In comparison, the sensitivity of the GOD/PPy/Pt electrode based on a flat PPy film was only approximately 330 nA/(m M cm 2 ) under the same conditions. The sensitivity of the microstructured GOD/PPy/Pt electrode could be increased to as high as approximately 2400 nA/(m M cm 2 ) at 37°C. The microstructured GOD/PPy/SS electrode had a sensitivity of approximately 550 nA/(m M cm 2 ) and a t 95% value of approximately 30 s at 15°C and 0.4 V. © 2005 Wiley Periodicals, Inc. J Appl Polym Sci 98: 2550–2554, 2005