Chemiluminescence Flow Injection Determination of Copper (II)
Afsaneh SafaviMohammad Reza Baezzat
Abstract
ABSTRACT A sensitive and selective flow injection chemiluminescence method for the determination of copper over the range of 0.5-7.5 ng is described. The method is based on quenching effect of copper (II) on the chemiluminescent reaction of dichlorofluorescein and hydrogen peroxide in alkaline media. Method development includes optimization of reagent concentrations and flow conditions. The optimized method yielded a detection limit (3σ) of 0.2 ng. The method is simple, fast, selective and precise and was used for the determination of copper in blood sera.
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