Immobilization of Candida antarctica lipase B on Polystyrene Nanoparticles

Abstract

Abstract Polystyrene (PS) nanoparticles were prepared via a nanoprecipitation process. The influence of the pH of the buffer solution used during the immobilization process on the loading of Candida antarctica lipase B (Cal‐B) and on the hydrolytic activity (hydrolysis of p ‐nitrophenyl acetate) of the immobilized Cal‐B was studied. The pH of the buffer solution has no influence on enzyme loading, while immobilized enzyme activity is very dependent on the pH of adsorption. Cal‐B immobilized on PS nanoparticles in buffer solution pH 6.8 performed higher hydrolytic activity than crude enzyme powder and Novozyme 435. magnified image