Impedimetric PSA Immunosensor: Prostate Specific Antigen Biosensor

Abstract

Regular check of blood prostate specific antigen (PSA) level is very important as it is related to prostate cancer. Hence, this study aims to build up a novel impedimetric PSA immumosensor (PSA-IMS) for accurate measurement of PSA. The impedimetric PSA-IMS was fabricated with PSA antibody immobilized on screen-printed carbon electrodes. Impedance measurement at a specific frequency ranges (22.80 Hz - 2.57 kHz) showed that the PSA-IMS has an excellent response range (6.25 - 400 ng/mL), with r ² >;0.9, which covers the normal physiological and pathological ranges of blood PSA levels. Intraclass correlation coefficient (ICC) showed that the PSA-IMS has excellent reliability and validit (ICC>;0.95). In conclusion, a simple and reliable PSA-IMS was developed and it is capable of precisely determining blood PSA levels in the range of pathological and normal physiological regions. in both the normal physiological and pathological ranges.