Dendrimer‐encapsulated Pt nanoparticles/polyaniline nanofibers for glucose detection

Abstract

Abstract A novel amperometric glucose biosensor based on self‐assembling glucose oxidase (GOx) and dendrimer‐encapsulated Pt nanoparticles (Pt‐DENs) on nanofibrous polyaniline (PANI) was described. PANI nanofibers were synthesized via an interfacial polymerization method. A sulfonated polyelectrolytes‐poly(sodium 4‐styrenesulfonate) (PSS) was used to form the negative PANI/sulfonated polyelectrolyte complex, which had good disperse in aqueous solution. GOx was immobilized on the PANI/PSS surface by alternatively assembling a cationic Pt‐DENs layer and an anionic GOx layer. The unique sandwich‐like layer structure (Pt‐DENs/GOx/Pt‐DENs/PANI/PSS) formed by self‐assembling provides a favorable microenvironment to keep the bioactivity of GOx and to prevent enzyme molecule leakage. The fabricated Pt‐DENs/GOx/Pt‐DENs/PANI/PSS electrode exhibited excellent response performance to glucose with a detection limit of 0.5 μ M , wide linear range from 10 μ M to 4.5 m M , short response time within 5 s, improved sensitivity of 39.63 μA/(m M cm 2 ), and good stability (85% remains after 20 days). © 2008 Wiley Periodicals, Inc. J Appl Polym Sci, 2008