Studies on Amino-hexoses. XI A Synthetic Substrate of Egg White Lysozyme: Phenyl 6-O-(2-Acetamido-2-deoxy-β-d-glucopyranosyl)-2-acetamido-3-O-(d-1′-carboxyethyl)-2-deoxy-β-d-glucopyranoside
Kazuhiko YamamotoYoshio Matsushima
Abstract
Abstract In the course of the study of the substrate specificity of egg white lysozyme, a phenyl glycoside of a disaccharide, N-acetyl-β-d-glucosaminyl(1→6)N-acetyl-muramic acid, was synthesized as a model substrate of the enzyme. Evidence for the 1→6 linkage between two component sugars was obtained by chemical and NMR studies. As a reference standard for a chromatographic investigation, 4-O-methyl muramic acid was prepared. The lysozyme action on the model substrate, if any, was found to be extremely weak.
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