A Glucose Biosensor Based on Immobilization of Glucose Oxidase in Electropolymerized o-Aminophenol Film on Platinized Glassy Carbon Electrode

Abstract

A high-performance amperometric glucose biosensor has been developed, based on immobilization of glucose oxidase in an electrochemically synthesized, nonconducting poly(o-aminophenol) film on a platinized glassy carbon electrode. The large microscopic surface area and porous morphology of the platinized glassy carbon electrode result in high enzyme loading, and the enzyme entrapped in the electrodeposited platinum microparticle matrix is stabler than that on a platinum disk electrode surface. The response current of the sensor is 20-fold higher than that of the sensor prepared with a platinum disk electrode of the same geometric area. The experiments showed that the high sensitivity of the sensor is due not only to the large microscopic area but also to the high efficiency of transformation of H(2)O(2) generated by enzymatic reaction to current signal on the platinized glassy carbon electrode. The response time of the sensor is <4 s, and its lifetime is >10 months.