Enhancement in production of recombinant two-chain Insulin Glargine by over-expression of Kex2 protease in Pichia pastoris

Suma Sreenivas; Sateesh M. Krishnaiah; Nagaraja Govindappa; Yogesh Basavaraju; Komal Kanojia; Niveditha Mallikarjun; N. Jayaprakash; Amarnath Chatterjee; Kedarnath N. Sastry

doi:10.1007/s00253-014-6052-5

JOURNAL ARTICLE

Enhancement in production of recombinant two-chain Insulin Glargine by over-expression of Kex2 protease in Pichia pastoris

Suma Sreenivas Sateesh M. Krishnaiah Nagaraja Govindappa Yogesh Basavaraju Komal Kanojia Niveditha Mallikarjun N. Jayaprakash Amarnath Chatterjee Kedarnath N. Sastry

Year: 2014 Journal: Applied Microbiology and Biotechnology Vol: 99 (1)Pages: 327-336 Publisher: Springer Science+Business Media

DOI: 10.1007/s00253-014-6052-5

Get Full-Text PDF Get Analytical Report

Keywords:

Pichia pastoris Proprotein convertase Insulin glargine Downstream processing Recombinant DNA Biochemistry Protease Trypsin Chemistry Pichia Escherichia coli Enzyme Biology Insulin Gene

Metrics

Cited By

1.66

FWCI (Field Weighted Citation Impact)

Refs

0.82

Citation Normalized Percentile

Is in top 1%

Is in top 10%

Citation History

Topics

Monoclonal and Polyclonal Antibodies Research

Health Sciences → Medicine → Radiology, Nuclear Medicine and Imaging

Bacterial Genetics and Biotechnology

Life Sciences → Biochemistry, Genetics and Molecular Biology → Genetics

Protein purification and stability

Life Sciences → Biochemistry, Genetics and Molecular Biology → Molecular Biology

Related Documents

JOURNAL ARTICLE

Extracellular Expression of Recombinant Kex2 Protease in Pichia pastoris BG10

Riyona Desvy Pratiwi Yuliawati Yuliawati Fitriagustiani Fitriagustiani Aminah Aminah Hariyatun Hariyatun Nuruliawaty Utami Dini Nurdiani Asrul Muhamad Fuad M Manfredi A Antunes L Jesus O De M Juliano L Juliano W Judice S De Y Huang Y Long S Li T Lin J Wu Y Zhang Y Lin B Granger M Outram P Solomon S Williams C Oliveira L Domingues D Waugh S Sreenivas S Krishnaiah N Govindappa Y Basavaraju K Kanojia N Mallikarjun J Natarajan A Chatterjee K Sastry A Caballero-Prez J Viader-Salvad A Herrera-Estala J Fuentes-Garibay M Guerrero-Olazarn N Govindappa M Hanumanthappa K Venkatarangaiah K Kanojia K Venkatesan A Chatterjee M Kusumanchi N Dave P Hazra S Tiwari S Yang Y Kuang H Li Y Liu X Hui P Li Z Jiang Y Zhou Y Wang A Xu G Luke M Ryan N Utami D Nurdiani E Hariyatun; Putro W Kusharyoto C Kielkopf W Bauer I Urbatsch M Tominaga K Nozaki D Umeno J Ishii A Kondo T Naumann K Sollenberger G Hao N Higgins A Vologodskii S Verma Z Qian S Adhya M Karbalaei S Rezaee H Farsiani R Aw K Polizzi Q Shen Z Yu X.-T Zhou S.-J Zhang S.-P Zou N Xiong Y.-P Xue Z.-Q Liu Y.-G Zheng S Zhao W Guan K Ma Y Yan J Ou J Zhang Z Yu J Wu Q Zhang G Lesage M Tremblay J Guimond G Boileau M Gmez-Gaviria N Lozoya-Prez M Staniszewska B Franco G Nio-Vega H Mora-Montes J Gao L Jiang J Lian Z Duman-zdamar B Binay L Wohlschlager F Csarman M Zrili B Seiboth R Ludwig M Naseem G Tajti A Gaspar T Szanto J Borrego G Panyi O Kondrashov P Kuzmin S Akimov P Aza G Molpeceres F De Salas S Camarero P Pu M Weissenborn

Journal: Biointerface Research in Applied Chemistry Year: 2022 Vol: 13 (4)Pages: 360-360

JOURNAL ARTICLE

Detection of recombinant Pichia pastoris producing glargine

Dudi Hardianto Efrida Martius Tina Rostinawati Anna Safarrida J I Royani Dihan Laziba Fahroziah Assyifa

Journal: IOP Conference Series Earth and Environmental Science Year: 2023 Vol: 1271 (1)Pages: 012089-012089

JOURNAL ARTICLE

Homologous Expression and Characterization of Pichia pastoris Kex2

Tong Wang Yan Xu Xiaowei Yu

Journal: Zhongguo shengwu gongcheng zazhi Year: 2019 Vol: 39 (1)Pages: 38-45

JOURNAL ARTICLE

Enhanced Production of Recombinant Secretory Proteins in Pichia pastoris by Optimizing Kex2 P1’ site

Yang Song Ye Kuang Hongbo Li Yue-Hong Liu Xiaoyan Hui Peng Li Zhiwu Jiang Yulai Zhou Yu Wang Aimin Xu Shiwu Li Pentao Liu Donghai Wu

Journal: PLoS ONE Year: 2013 Vol: 8 (9)Pages: e75347-e75347

JOURNAL ARTICLE

Recombinant Expression of Human Enteropeptidase Light Chain in Pichia pastoris

Eliot T. Smith Mallory McGhee David A. Johnson

Journal: The FASEB Journal Year: 2012 Vol: 26 (S1)