Determination of Levamisole in Livestock Products Using High Performance Liquid Chromatography.

Miho Sakamoto; Kazue Takeba; Kenji Fujinuma; Katsuhiko JIMBO; Tomoyuki Miyazaki

doi:10.3358/shokueishi.43.6

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JOURNAL ARTICLE

Determination of Levamisole in Livestock Products Using High Performance Liquid Chromatography.

Miho Sakamoto Kazue Takeba Kenji Fujinuma Katsuhiko JIMBO Tomoyuki Miyazaki

Year: 2002 Journal: Food Hygiene and Safety Science (Shokuhin Eiseigaku Zasshi) Vol: 43 (1)Pages: 6-9 Publisher: Japanese Society for Food Hygiene and Safety

DOI: 10.3358/shokueishi.43.6

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Abstract

高速液体クロマトグラフィーによる畜産物(筋肉,肝臓,腎臓,脂肪)中のレバミゾールの分析法を検討した.試料をアルカリ性条件下,酢酸エチルで抽出した後,塩酸溶液で再抽出した.抽出液をSCXカートリッジカラムに負荷し,水及びメタノールで洗浄後,アンモニア水-メタノール混液で溶出した.溶出液を減圧濃縮乾固し,残留物を移動相に溶解し,フォトダイオードアレイ検出器付高速液体クロマトグラフにより測定した.各試料に0.01又は0.10μg/gとなるようにレバミゾールを添加した場合の回収率は 78.3~99.8% であり,検出限界は0.005μg/gであった.

Keywords:

Levamisole Chromatography Chemistry High-performance liquid chromatography Livestock Biology Ecology Zoology

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Pharmacological Effects and Assays

Life Sciences → Agricultural and Biological Sciences → Animal Science and Zoology

Pesticide Residue Analysis and Safety

Life Sciences → Agricultural and Biological Sciences → Food Science

Analytical Chemistry and Chromatography

Physical Sciences → Chemistry → Spectroscopy

Determination of Levamisole in Livestock Products Using High Performance Liquid Chromatography.

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