Determination of methyltestosterone using flow injection with chemiluminescence detection

Abstract

Abstract Methyltestosterone (MT) enhanced the chemiluminescence of potassium permanganate–sodium thiosulphate system in sulphuric acid medium, and this was used as the basis of a novel flow‐injection chemiluminescence method for the determination of methyltesterone. Under optimum conditions, the linear response of MT was in the range 3.0 × 10 −8 –8.0 × 10 −6 g[sol ]mL, and the detection limit for determination of MT was 1.0 × 10 −8 g[sol ]mL. The method was applied to the analysis of fish with recovery in the range 88.0–113.8% with an average RSD of 1.66% ( n = 8). Copyright © 2005 John Wiley & Sons, Ltd.