Measurement of nicotinamide adenine dinucleotide & nicotinamide adenine dinucleotide phosphate in tomato leaves

Abstract

As part of an investigation of the growth of tomato plants supplied with either nitrate or ammonium nitrogen (6), leaves were assayed for NAD4 and NADP. Anderson and Vennesland (1) assayed NAD and NADP in various green tissues and reported recoveries between 50 % and 85 % of NAD and NADP added to spinach leaf extracts. Very low levels of NAD and NADP were recorded for tomato leaf extracts. These low levels were attributed to losses of NAD and NADP in the additional purification steps necessary with tomato leaf material for removal of other compounds absorbing at 340 mIy. The method reported here provides an improved procedure for the assay of NAD and NADP in tomato leaf extracts.