Determination of sulphite using an immobilized enzyme with flow injection chemiluminescence detection

Abstract

Abstract A flow injection method is reported for the determination of sulphite‐based on chemiluminescent detection. Hydro‐gen peroxide is produced from sulphite using on‐line covalently bound immobilized sulphite oxidase packed in a mini‐column, which was mixed downstream and detected via cobalt(II)‐catalysed chemiluminescent oxidation of luminol. The limit of detection (2 × standard deviation of the blank) was 1 × 10 −3 mmol/L with sample throughput 60 h −1 . The calibration data was linear over the range of 0.2–1.0 mmol/L with relative standard deviation ( n = 4) in the range 0.9–2.0%. Copyright © 2004 John Wiley & Sons, Ltd.