A Glucose Biosensor Based on Immobilization of Glucose Oxidase in Chitosan Network Matrix

Abstract

A simple and promising glucose biosensor was constructed by successful entrapment of glucose oxidase (GOD) into chitosan matrix, which was cross-linked with glutaraldehyde (GA). This material provided good biocompatibility and the stabilizing microenvironment around the enzyme. The morphologies and properties of chitosan and chitosan/GOD were characterized by FTIR, UV-Vis and SEM techniques. This biosensor exhibited a fast amperometric response to glucose. The linear range for glucose determination was from 1×10−5 to 3.4×10−3 mol·L−1, with a detection limit of 5×10−6 mol·L−1 based on S/N=3. The biosensor could retaCin ca. 90% of its original activity after two weeks of storage under dry conditions at 4 °C.