Multiplex-PCR for differentiation of Mycobacterium bovis from Mycobacterium tuberculosis complex
F.L.E. SposittoPaula Aline Zanetti CampanerutLuciana Dias GhiraldiClarice Q.F. LeiteMário Hiroyuki HirataRosário Dominguez Crespo HirataVera Lúcia Dias SiqueiraRosilene Fressatti Cardoso
Abstract
We evaluated a multiplex-PCR to differentiate Mycobacterium bovis from M. tuberculosis Complex (MTC) by one step amplification based on simultaneous detection of pncA 169 C > G change in M. bovis and the IS6110 present in MTC species. Our findings showed the proposed multiplex-PCR is a very useful tool for complementation in differentiating M. bovis from other cultured MTC species.
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