SNP detection based on temperature-controllable EWOD digital microfluidics system

Abstract

This study based on the technique, "Electrowetting on Dielectric (EWOD)," with the micro-heaters designed in EWOD microfluidics system for temperature controlling in biomedical reaction. On this platform, fluorescence magnetic beads (MBs) were used as carriers for single-nucleotide polymorphism (SNP) detection. In human genome, SNPs are responsible for the variations between individuals. The genome of a person comprise specific SNP could result in responsiveness to drug therapies and sensitivity of certain disease. Therefore, SNP is considered as one of the keys to predict the affect in pharmacogenomic medicine. In this study, SNP detection has been demonstrated on this thermal controllable EWOD platform in order to approach the goal of "lab-on-a-chip".