Pectinesterase Inhibitor in Rubbery Banana ( Musa sapientum L. )

Abstract

Abstract Pectinesterase (PE) (7 U/mL), extracted from ripe and soft banana flesh (RSBF), was prepared with a ratio of 4 parts (1:4) 2% NaCl. PE activity in the 2% NaCl extracted from homogenized RSBF and rubbery banana flesh (RBF) were 7 and 2 U/mL, respectively, during the 16 h PE extraction, suggesting the existence of the PE inhibitor (PEI) in RBF. Crude PEI extracts from RBF and RSBF exhibited about 80% and 17% inhibitory ratios, respectively, on banana PE activity. Crude PEI from RBF, boiled for 30 to 180 min, showed a 79 to 82% inhibitory ratio of banana PE activity.