Hyperthermia HeLa Cell Treatment with Silica-Coated Manganese Oxide Nanoparticles

Abstract

The effect of a high frequency alternating magnetic field on HeLa tumour\ncells incubated with ferromagnetic nanoparticles of manganese oxide perovskite\nLa0.56(SrCa)0.22MnO3 have been studied. The particles were subjected to a size\nselection process and coated with silica to improve their biocompatibility. The\ncontrol assays made with HeLa tumour cells showed that cell survival and growth\nrate were not affected by the particle internalization in cells, or by the\nelectromagnetic field on cells without nanoparticles. However, the application\nof an alternating electromagnetic field to cells incubated with this silica\ncoated manganese oxide induced a significant cellular damage that finally lead\nto cell death by an apoptotic mechanism. Cell death is triggered even thought\nthe temperature increase in the cell culture during the hyperthermia treatment\nis lower than 0.5 C.\n