Cloning and Characterization of the Crystal Protein-Encoding Gene of Bacillus thuringiensis subsp. yunnanensis
P.R. BalasubramanianR JayakumarPrashant B. ShambharkarN. UnnamalaiShunmugiah Karutha PandianN. Selvamuthu KumaraswamiR. IlangovanVaithilingam Sekar
Abstract
ABSTRACT Molecular cloning and characterization of a novel cry gene, cry32Aa , of Bacillus thuringiensis subsp. yunnanensis was carried out. The Cry32Aa protein was predicted to have a molecular mass of 139.2 kDa and was found to have an unusual 42-amino-acid-long tail at the C terminus. The cry32Aa gene was localized on the 103-MDa plasmid of the organism. Bioassays showed no toxicity against several moths and mosquitoes. However, it exhibited weak toxicity against larvae of the diamondback moth, Plutella xylostella .
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