JOURNAL ARTICLE

A Mediator‐Free Bienzyme Amperometric Biosensor Based on Horseradish Peroxidase and Glucose Oxidase Immobilized on Carbon Nanotube Modified Electrode

Yanli YaoKwok‐Keung Shiu

Year: 2008 Journal:   Electroanalysis Vol: 20 (19)Pages: 2090-2095   Publisher: Wiley

Abstract

Abstract Multiwalled carbon nanotube (CNT) modified glassy carbon electrode immobilized with horseradish peroxidase (HRP) in Nafion coating showed direct electron transfer between HRP enzyme and the CNT‐modified electrode. A mediator‐free bienzyme glucose biosensor based on horseradish peroxidase and glucose oxidase was constructed. The bienzyme biosensor exhibited a high sensitivity for glucose detection at zero applied potential.

Keywords:
Horseradish peroxidase Biosensor Glucose oxidase Amperometry Nafion Chemistry Carbon nanotube Peroxidase Electrode Immobilized enzyme Chromatography Nanotechnology Materials science Organic chemistry Electrochemistry Enzyme Biochemistry

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42
Cited By
4.56
FWCI (Field Weighted Citation Impact)
38
Refs
0.95
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Citation History

Topics

Electrochemical sensors and biosensors
Physical Sciences →  Engineering →  Electrical and Electronic Engineering
Advanced biosensing and bioanalysis techniques
Life Sciences →  Biochemistry, Genetics and Molecular Biology →  Molecular Biology
Analytical Chemistry and Sensors
Physical Sciences →  Chemical Engineering →  Bioengineering
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