Antibody‐based surface plasmon resonance detection of intact viral pathogen

Abstract

Abstract Surface plasmon resonance (SPR) technique was used to directly detect an intact form of insect pathogen: the baculovirus, Autographa californica multiple nuclear polyhedrosis virus (AcMNPV). An SPR sensor chip with three bio‐functional layers was used to detect the intact AcMNPV: amine‐reactive crosslinker with a disulfide bond that chemisorbs to gold film, Protein A, and a mouse IgG monoclonal antibody raised against a surface protein of the target viral pathogen. A two‐channel (reference & test) micro‐fluidic SPR system is used for reliable measurement. Bio‐specific response to the AcMNPV is compared with the response for tobacco mosaic virus (TMV) as control. Successive exposure of the sensor chip to both viruses verifies a specific response to AcMNPV. This serves as a prerequisite to the development of a new type of viral pathogen detection sensors. © 2006 Wiley Periodicals, Inc.