Enzyme electrode for glucose based on the quinoprotein glucose dehydrogenase

Abstract

Glucose dehydrogenase (E.C. 1.1.99.17) has been used to produce an amperometric enzyme electrode for glucose. With phenazine ethosulphate as the electron mediator, a linear response for up to 0.8 mM glucose was obtained with response times of 3–5 min. 2,6-Dichlorophenolindophenol as mediator gave long (> 30 min) response times, but the measurement time was reduced to 30 s by monitoring the first-order differential of the response; the derivatised signal allowed temporal separation of the enzyme-mediated signal from electrochemical interference. Whole blood glucose measurements were correlated with a spectrophotometric method based on glucose oxidase (y= 0.84x + 0.67, r= 0.963, n= 36).