Fabrication of CNT/Ferrocene/Glucose Oxidase/Chitosan-Layered Bioanode for Glucose/Oxygen Biofuel Cells

Abstract

Abstract An enzyme-modified electrode was fabricated by entrapping glucose oxidase (GOx) and ferrocene (Fc) onto a multiwall carbon nanotube (MWCNT)-coated electrode. The MWCNT, Fc, GOx, and chitosan (CHI) were sequentially coated on a glassy carbon electrode. The MWCNT/Fc/GOx/CHI electrode was characterized by scanning electron microscopy (SEM), and cyclic voltammetry (CV). The prepared electrode exhibited good electrochemical performance for the glucose analysis with a linear range of 0–60 mM glucose. It was found that the MWCNT film on the electrode remarkably enhanced the performance of the electrode. The MWCNT/Fc/GOx/CHI electrode was integrated with a bilirubin oxidase-immobilized cathode for a biofue cell application. The maximum power density at a glucose concentration of 10 mM was 13 μW/cm2 at a cell voltage of 0.19 V. The results of this study indicate that the MWCNT/Fc/GOx/CHI electrode could be applied in the development of biofuel cells and bisensors. Keywords: Bioanodebiofuel cellschitosanferroceneglucose oxidaseglucose sensormutiwalled carbon nanotubes Acknowledgment This research was supported by the National Research Foundation of Korea (NRF) funded by the Ministry of Education, Science and Technology (2009–0093906).