Fluorescence lifetime imaging using light-emitting diodes

Gordon T. Kennedy; Daniel S. Elson; Ian Munro; V. Poher; P. M. W. French; Mark A. A. Neil

doi:10.1117/12.701088

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Fluorescence lifetime imaging using light-emitting diodes

Gordon T. Kennedy Daniel S. Elson Ian Munro V. Poher P. M. W. French Mark A. A. Neil

Year: 2007 Journal: Proceedings of SPIE, the International Society for Optical Engineering/Proceedings of SPIE Vol: 6443 Pages: 644315-644315 Publisher: SPIE

DOI: 10.1117/12.701088

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Abstract

We demonstrate fluorescence lifetime imaging using cheap, high power light emitting diodes as excitation sources. Both time domain and frequency domain fluorescence lifetime imaging techniques have been implemented at wavelengths spanning the range 450 nm to 640 nm.

Keywords:

Fluorescence Optoelectronics Materials science Diode Fluorescence-lifetime imaging microscopy Light-emitting diode Excitation Wavelength Optics Excitation wavelength Range (aeronautics) Physics

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Advanced Fluorescence Microscopy Techniques

Life Sciences → Biochemistry, Genetics and Molecular Biology → Biophysics

Semiconductor Lasers and Optical Devices

Physical Sciences → Engineering → Electrical and Electronic Engineering

Semiconductor Quantum Structures and Devices

Physical Sciences → Physics and Astronomy → Atomic and Molecular Physics, and Optics

Fluorescence lifetime imaging using light-emitting diodes

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