Enzyme Immobilized Biosensor for L-Lactate

Abstract

L-lactate has an important role on biochemical process such as producing ATP, causingcramps in muscle. The lactate consumption in various proposes is increasing in human life. It isproduced by fermentation process with microorganism. Measuring L-lactate concentration by a rapidand simple method is a great need in clinical and food industries. Analysis of enzyme reaction on Llactatewas performed with L-LDH (L-lactate dehydrogenase), and NAD+ (nicotinamide adeninedinucleotide). To avoid enzyme reverse reaction of L-LDH, GPT (glutamic-pyruvic transaminase) andL-glutamate were supplied. Substances were immobilized with polyacrylamide gel on tip of glasselectrode. Calibration with L-lactate and output signal was performed. The relation between the Llactate(%) and the signal from the electrode (mV) was y=0.0719x-16.576. The calibration had acorrelation coefficient of R2=0.9959 and R2=0.9919 when it was validated.