Superresolved femtosecond laser nanosurgery of cells

Matthias Pospiech; Moritz Emons; Kai Kuetemeyer; Alexander Heisterkamp; Uwe Morgner

doi:10.1109/cleoe.2011.5943235

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Superresolved femtosecond laser nanosurgery of cells

Matthias Pospiech Moritz Emons Kai Kuetemeyer Alexander Heisterkamp Uwe Morgner

Year: 2011 Pages: 1-1

DOI: 10.1109/cleoe.2011.5943235

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Abstract

We report on femtosecond nanosurgery of fluorescently labeled structures in cells with a spatially superresolved laser beam. The focal spot width is reduced using phase filtering applied with a programmable phase modulator. A comprehensive statistical analysis of the resulting cuts demonstrates an achievable average resolution enhancement of 30 %.

Keywords:

Optics Femtosecond Laser Materials science Microscope Microscopy Optical microscope Diffraction Fluorescence microscope Laser ablation Ablation Fluorescence Scanning electron microscope Physics

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Laser Material Processing Techniques

Physical Sciences → Engineering → Computational Mechanics

Advanced Fluorescence Microscopy Techniques

Life Sciences → Biochemistry, Genetics and Molecular Biology → Biophysics

Nonlinear Optical Materials Studies

Physical Sciences → Engineering → Biomedical Engineering

Superresolved femtosecond laser nanosurgery of cells

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