GAS LIQUID CHROMATOGRAPHY OF WORT AND BEER CARBOHYDRATES II. QUANTITATIVE DETERMINATION OF WORT, BEER, AND BREWING SYRUP CARBOHYDRATES

Abstract

A method for the preparation and gas chromatography of the trimethylsilyl derivatives of carbohydrates was applied to the quantitative analysis of carbohydrates in worts, beers, and brewing syrups. The derivatives can be easily and quickly prepared from lyophilized worts or beers and aliquots of the reaction mixtures can be injected directly into the gas chromatograph. Gas chromatographic separations of the mono- to tetra-saccharide fractions were achieved in less than thirty minutes through the use of linear temperature programming. Recoveries of carbohydrates added to wort ranged from 97 to 105%. Quantitative data for replicate wort analyses showed relative standard deviations of from 1·7% for glucose to 5·3% for maltose. The silylation reaction can be applied to the volatilization and subsequent study of a wide variety of compounds occurring in brewing materials.