SENSITIVE L-CYSTEINE AMPEROMETRIC SENSOR BASED ON A GLASSY CARBON ELECTRODE MODIFIED BY MnO₂NANOPARTICLES

Abstract

A sensitive amperometric sensor for the determination of L-cysteine was fabricated by electrodeposition of modified MnO2 nanoparticles on a glassy carbon electrode. The morphology of the nanoparticles was characterized by scanning electron microscopy. Cyclic voltammetry was applied to investigate the mechanism of film formation and the electrochemical properties of the MnO2 nanoparticle-modified electrode. The results of electrochemical experiments showed that the modified electrode had a favorable catalytic ability for the electrochemical oxidation of L-cysteine. Under optimized conditions, the MnO2 nanoparticle-modified electrode exhibited a linear dependence on the concentration of L-cysteine from 1.0 × 10−6 to 6.4 × 10−4 M, and a detection limit of 8.0 × 10−7 M (signal/noise = 3). The modified electrode was highly resistant towards typical inorganic salts and some biomolecules. In addition, the sensor was applied for the determination of L-cysteine in human serum with high accuracy, demonstrating its potential for practical applications.