Antioxidative activities of grape (Vitis vinifera) seed extracts obtained from different varieties grown in Turkey

Abstract

Summary In this study, total phenolic contents and antioxidant activities of grape seed extracts obtained from twelve different grape seeds from common varieties grown in Turkey were determined. Grape seeds were extracted with 70% acetone and extraction yield of grape seed were calculated. The total phenolic content of grape seed extracts were determined by the Folin‐Ciocalteu procedure and ranged from 33 945 to 58 730 mg per 100 g extract as gallic acid equivalent. Antioxidant activities of grape seed extracts with two different free radical scavenging methods, ABTS [2,2/‐azinobis (3‐ethylbenzothiazoneline‐6‐sulfonic acid)] and DPPH (2,2‐diphenyl‐picrylhydrazyl) assays, using Trolox equivalent as standards, were investigated. Grape seed extracts exhibited antioxidant activities 2.46–4.14 and 3.55–5.76 [Trolox equivalent antioxidant capacities (TEAC) mg −1 extracts] in ABTS and DPPH assays, respectively. Compared with varieties, Muskule extracts exhibited the lowest total phenolic content, TEAC ABTS and TEAC DPPH value while Narince extracts had the highest total phenolic content and TEAC DPPH value, and Alphonse Lavalleé had the highest TEAC ABTS value. Total phenolic content showed that there is a significant correlation with TEAC DPPH ( r = 0.7974, P ≤ 0.001) and TEAC ABTS values ( r = 0.4860, P ≤ 0.05).